Pseudomonas fluorescens lab report

Pseudomonas fluorescens symptoms

Depending on the quality and solubility of the carbon source tested, vary the concentration accordingly. Nucleic Acids Res. While not suspected of being an etiologic agent of pulmonary disease, we recently reported that P. J Gen Virol. The mannitol salt agar pate returned with four perfectly isolated colonies. However, we recommend only restreaking from this original agar plate to start fresh plates as needed within that week. While significantly less virulent than P. Altogether, these reports and others are beginning to highlight a far more common, and potentially complex, interaction between humans and P. A Simmons citrate and SIM tube tests were executed to narrow down the list of possible gram negative bacterium.

The bacterium can be spread in hospitals from such a thing as simple as health care workers not washing their hands, or from a piece of equipment that was not properly cleaned.

Altogether, these reports are beginning to highlight a far more common, intriguing, and potentially complex association between humans and P. Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development.

This colony was grown on a milk agar plate to gram stain once again to reconfirm that it is in fact isolated and tests on the bacterium could continue.

Pseudomonas aeruginosa lab identification

Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development. Pseudomonas Genome Database: facilitating user-friendly, comprehensive comparisons of microbial genomes. The mannitol salt agar pate returned with four perfectly isolated colonies. Finally, urea and casein tests were performed to determine which bacterium was present. The amount of pigment produced varies between strains and a sweet grape-like odor, caused by 2-aminoacetophenone, can sometimes be smelled when the bacteria is grown on a rich medium. I also had trouble getting the correct bacteria to grow on the correct plates. After the initial plate is one week old, we recommend retreaking a fresh plate from the frozen stock rather than conducting serial passage of the strain on plates. The mannitol salt agar plate used to grow the gram positive bacterium returned with one yellow opaque isolated colony. However, this final filtration step eliminates potential contamination issues from long-term stocks and is good practice particularly when studying mutants that may grow slower than wild-type for a given carbon source. Mol Microbiol. Genome diversity of Pseudomonas aeruginosa PAO1 laboratory strains. J Microbiol Methods. Due to the fact that the gram negative bacterium was isolated a lawn was created on a mannitol salt agar plate from the original broth, which selects for gram positive bacteria to grow. The plate must have been contaminated as gram negative bacteria never should have proliferated on a mannitol salt agar plate. The instructor explained that the mannitol salt agar plate gram stain should have returned gram positive rods and not coccyx, which narrowed the list down to the first two bacteria that were eliminated.

Microbiology of sputum from patients at cystic fibrosis centers in the United States. Baader and C. Pulmonary infections in patients with cystic fibrosis.

pseudomonas fluorescens ppt

The plate must have been contaminated as gram negative bacteria never should have proliferated on a mannitol salt agar plate. While not suspected of being an etiologic agent of pulmonary disease, we recently reported that P.

These microorganisms range from deadly to vital for life and health to continue. A gram stain test was performed and purple rods were revealed, a confirmed gram positive result. The results from the second streak plate were positive and showed one perfectly isolated colony.

Pseudomonas fluorescens identification

The gram stain for the alternate revealed red rods, a confirmed gram negative result. Evaluation of flagella and flagellin of Pseudomonas aeruginosa as vaccines. The mannitol salt agar plate used to grow the gram positive bacterium returned with one yellow opaque isolated colony. However, while far less virulent than P. They are not required for growth on glucose and they are not part of the media from the original citation. Antibiotics for emerging pathogens. However, we recommend only restreaking from this original agar plate to start fresh plates as needed within that week. The large genome size and complexity reflect the physiologic adaptability of P. The road to discovering both unknown bacteria given by the instructor was a rocky one; seemingly countless trials and errors later, a conclusion was reached for both. A min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: Application for DNA fragment transfer between chromosomes and plasmid transformation. However, this final filtration step eliminates potential contamination issues from long-term stocks and is good practice particularly when studying mutants that may grow slower than wild-type for a given carbon source. The gram stain from one of the more isolated colonies revealed purple coccyx, a confirmed gram positive result, which narrowed the list of possibilities down to three bacteria. A gram stain was performed on this colony and revealed red rods, a confirmed gram negative result.

The fourth streak plate returned with no isolated colonies so a fifth and final streak plate was created. Culture medium for Enterobacteria.

The bacterium acts as an opportunistic pathogen and therefore rarely causes disease in healthy people.

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